Simple Method for Culturing Anaerobes

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چکیده

منابع مشابه

Simple method for culturing anaerobes.

A simple, effective method is needed for growing obligate anaerobes in the clinical laboratory. This report describes a pre-reduced anaerobic bottle that can be taken to the bedside for direct inoculation, provides a flat agar surface for evaluation of number and morphology of colonies, and can be incubated in conventional bacteriological incubators. Each anaerobic culture set consisted of two ...

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Simple and convenient method for culturing anaerobic bacteria.

A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic inc...

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Pipette count method for obligate anaerobes.

The routine counting of obligate anaerobic bacteria is a serious problem in many laboratories. Reliable anaerobic chambers are either expensive or messy and cumbersome. Furthermore, the exposure of certain genera, notably Bacteroides and some spirochetes, to air may kill the organisms. The following technique does not require an anaerobic chamber, does not expose the organism to atmospheric oxy...

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Validation of performance of plastic versus glass bottles for culturing anaerobes from blood in BacT/ALERT SN medium.

To validate performance, we compared the new plastic BacT/ALERT (bioMérieux, Durham, NC) SN bottle to the current glass SN bottle with samples of blood obtained for culture from adults and found them comparable for both recovery and speed of detection of microorganisms. We conclude that the safety advantage of plastic bottles can be achieved without compromising performance.

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A convenient Simple Method for Synthesis of Meta-iodobenzylguanidine (MIBG)

Radioiodinated meta iodobenzylguanidine (MIBG) is one of the important radiopharmaceuticals in Nuclear Medicine. [123/131I]MIBG is used for imaging of Adrenal medulla, studying heart sympathetic nerves, treatment of pheochromacytoma and neuroblastoma. For clinical application, radioiodinated MIBG is prepared through isotopic exchange method, which includes replacement of radioactive iodine in a...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1973

ISSN: 0003-6919

DOI: 10.1128/am.25.2.216-221.1973